Self-organization mechanism of adaptive colony size sensing in ants

Social insects such as ants and termites are superorganisms, and traits of a colony change in a manner similar to the growth of an individual. The most common pattern is that reproductive castes are produced only when the colony size exceeds a certain threshold, which is well known to be adaptive. This means that social insects can “sense” their own colony size. However, how they achieve this even without visual information in a dark environment was yet largely unknown. We empirically tested the self-organization hypothesis on the proximate mechanism using ant colonies. In Diacamma colonies the monogynous queen is known to increase the effort devoted to queen pheromone–transmission behaviour (patrolling) as the colony grows, as if she perceives colony size. The negative feedback hypothesis assumes that through repeated physical contacts with workers the queen monitors the physiological state (fertility) of workers and increases her patrolling effort when she encounters more fertile workers. Supporting this hypothesis, we found that queens increased patrol effort in response to a higher ratio of fertile workers under the experimental condition of constant colony size. Furthermore, supplementary experiments suggested that cuticular hydrocarbons can mediate the observed queen–worker communication of fertility state. However, when the colony size exceeds a certain value, information transmission fails, resulting in the production of the next generation of reproductive caste. Such a self-organising mechanism of sensing colony size may also operate in other social insects living in small colonies.