Plasticity in the endogenous rhythms and the adaptation to the tidal environment in a freshwater snail

Organisms have diverse biological clocks synchronized with environmental cycles　depending on their habitats. The change in endogenous rhythms could contribute to range expansion in a novel rhythmic environment. For example, the Anticipation of tidal changes has driven the evolution of circatidal rhythms in some marine species. I am interested in the genetic and non-genetic changes in the biological rhythms and adaptation to tidal environments in the freshwater snail, Semisulcospira reiniana. Chronobiological analyses of behavior and gene expression revealed that snails had habitat-specific endogenous rhythms: individuals in a nontidal population showed the circadian rhythm while those in a tidal population showed the circadian and circatidal rhythms. The entrainment to the simulated tidal cycles increased the strength of circatidal activity only in snails in a tidal population. Although the circatidal rhythms in the transcriptome were clearer in individuals entrained to tidal cycles, the number of circatidal rhythmic transcripts was greater in a tidal population than in a nontidal population. These results suggest biological rhythms in the snails plastically change at the molecular level, but the strength of circatidal rhythm is different between populations. Finally, transcriptome-wide population genetic analysis revealed that these two populations can be clearly distinguished genetically, though the genetic distance was very small. Thus, genetic differentiation in biological rhythms could result from the evolution of a small number of genes. These findings suggest that adaptive plasticity and genetic changes in the biological rhythms play an important role in coping with tidal environments.